aav6 vectors Search Results


raav6 virus  (PackGene Biotech lnc)
95
PackGene Biotech lnc raav6 virus
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Raav6 Virus, supplied by PackGene Biotech lnc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/AAV6+Vector+Production/pmc10850376-39-1-6
Average 95 stars, based on 1 article reviews
raav6 virus - by Bioz Stars, 2026-09
95/100 stars
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aav6 gfp  (Vector Biolabs)
95
Vector Biolabs aav6 gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/AAV6-GFP/us11920158-335-3-4
Average 95 stars, based on 1 article reviews
aav6 gfp - by Bioz Stars, 2026-09
95/100 stars
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control aav6  (Vector Biolabs)
95
Vector Biolabs control aav6
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Control Aav6, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/AAV6-GFP-U6-shRNA/pmc09226085-74-9-13
Average 95 stars, based on 1 article reviews
control aav6 - by Bioz Stars, 2026-09
95/100 stars
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aav6 cag cre gfp  (Vector Biolabs)
95
Vector Biolabs aav6 cag cre gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Cag Cre Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/AAV6-CAG-GFP/pm34453065-127-5-6
Average 95 stars, based on 1 article reviews
aav6 cag cre gfp - by Bioz Stars, 2026-09
95/100 stars
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aav6 2 camkiiα ert2 cre  (Vector Biolabs)
95
Vector Biolabs aav6 2 camkiiα ert2 cre
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 2 Camkiiα Ert2 Cre, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/AAV6-Cre/pmc11193045-16-0-2
Average 95 stars, based on 1 article reviews
aav6 2 camkiiα ert2 cre - by Bioz Stars, 2026-09
95/100 stars
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aav cre gfp  (Vector Biolabs)
90
Vector Biolabs aav cre gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav Cre Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/AAV6-Cre-GFP/pmc08895126-295-0-9
Average 90 stars, based on 1 article reviews
aav cre gfp - by Bioz Stars, 2026-09
90/100 stars
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aav6 cmv con  (Vector Biolabs)
92
Vector Biolabs aav6 cmv con
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Cmv Con, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/AAV6+Null/pm37371128-69-30-33
Average 92 stars, based on 1 article reviews
aav6 cmv con - by Bioz Stars, 2026-09
92/100 stars
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aav6-shklf6  (Shanghai GenePharma)
90
Shanghai GenePharma aav6-shklf6
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Shklf6, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/aav6+recombinant+vector/pm40234972-173-0-6
Average 90 stars, based on 1 article reviews
aav6-shklf6 - by Bioz Stars, 2026-09
90/100 stars
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aav6 vectors  (Obio Technology Corp Ltd)
86
Obio Technology Corp Ltd aav6 vectors
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Vectors, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/aav6+vectors/pmc13032692-37-0-5
Average 86 stars, based on 1 article reviews
aav6 vectors - by Bioz Stars, 2026-09
86/100 stars
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aav6 2ff vector  (Yeasen Biotechnology)
86
Yeasen Biotechnology aav6 2ff vector
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 2ff Vector, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/2ff+aav6+vector/pm41402336-267-15-28
Average 86 stars, based on 1 article reviews
aav6 2ff vector - by Bioz Stars, 2026-09
86/100 stars
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virus strains top10 fischer scientific  (Merck & Co)
86
Merck & Co virus strains top10 fischer scientific
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Virus Strains Top10 Fischer Scientific, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6+vectors/aav2+aav6+aav9+biolabs+biolabs+biolabs+cag+cag+chemicals+custom+custom+custom+maif1+order+order+order+rfp+rfp+rfp+strains+vector+vector+vector+virus/pm31353185-134-7-16
Average 86 stars, based on 1 article reviews
virus strains top10 fischer scientific - by Bioz Stars, 2026-09
86/100 stars
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Image Search Results


Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Journal: Frontiers in Cell and Developmental Biology

Article Title: In situ correction of various β-thalassemia mutations in human hematopoietic stem cells

doi: 10.3389/fcell.2023.1276890

Figure Lengend Snippet: Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Article Snippet: The rAAV6 virus was purchased from PackGene, and the titer value was determined to be 1E+13 GC/mL by quantitative PCR detection.

Techniques: Derivative Assay, Selection, Marker, Flow Cytometry, Expressing